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Molecular.Biology.Insights.Oligo.v7.53
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  • 更新时间:2011-03-10 21:16:10
  • 软件类别:国外软件 / 其它软件
  • 软件语言:英文
  • 授权方式:商业版
  • 联系方式:ygqh@21cn.com
  • 官方主页:Home Page
  • QQ、手机:没有预览图片
  • 运行环境:Win2000/WinXP

软件简介

OLIGO 是一个从序列文件中搜索和选择寡核苷酸的多功能程序,应用于聚合酶链反应(PCR),DNA测序,定点突变及各种各样的杂交研究。OLIGO根据最近邻热力学值计算寡核苷酸的杂交温度和二级结构。 这个好工具也用于构建合成基因,在已合成的基因中发现适当的序列引物,发现和多元化一致的引物和探针, 甚至发现蛋白质潜在的限制位点。
 

  OLIGO Primer Analysis Software is the essential tool for designing and       
  analyzing sequencing and PCR primers, synthetic genes, and various kinds of  
  probes including siRNA and molecular beacons.                                
  Based on the most up-to date nearest neighbor thermodynamic data, Oligo's    
  search algorithms find optimal primers for PCR, including TaqMan, highly     
  multiplexed, consensus or degenerate primers.  Multiple file batch           
  processing is possible. It is also an invaluable tool for site directed      
  mutagenesis.                                                                 
  For each primer or primer pair, Oligo's various analysis windows show a      
  multitude of useful data, such as DNA and RNA secondary structure, dimer     
  formation, false priming and homology, internal stability, composition and   
  physical properties.  With Oligo you can analyze open reading frames down to 
  predicted molecular weight and pKa of proteins, and search for restriction   
  enzyme sites, not only in DNA but also in reverse-translated proteins.       
  The first version of the software appeared on the market in 1989. It went    
  through several modifications, and the last one, the change from version 6   
  to 7, was the most comprehensive. Oligo 7 can automatically select multiplex 
  primers, process sequence files in batch modes, automatically design PCR     
  primers to cover multiple DNA regions in just one search and automatically   
  find primer/probe sets for real time PCR or finds nested primers sets. Oligo 
  search protocols (scoring system) may be customized in detail, so you may    
  optimize the results according to your specific needs.     

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